Process for preparation of wide-pore adsorbent for use in chromatography

ABSTRACT

A process for preparing a wide-pore adsorbent adapted for use in chromatography, comprising mixing finely dispersed non-porous silica having a specific surface area S of 35 to 380 m2/g with water followed by drying the aqueous suspension at a temperature within the range of 100 to 450*C to give a silica xerogel and particulating thereof. The xerogel, prior to particulation or thereafter, is subjected to the hydrothermal treatment in an autoclave at a temperature within the range of from 100 to 380*C and under a pressure of 1 to 300 atm., followed by drying at a temperature of 100 to 300*C. The above-described process makes possible the preparation of an adsorbent with larger pores as compared to adsorbents prepared from finely dispersed non-porous silica and produced by conventional processes. The process according to the present invention makes it possible to produce an adsorbent having a specific surface area within the range of from 2 to 280 m2/g and an average pore diameter of from 230 to 30,000 A, while retaining a high pore volume of 0.9 to 1.9 cm3/g. Such adsorbent is useful in chromatography for the purposes of separation, purification and analysis of a wide range of substances: from low- and moderately-boiling compounds up to polymers, and more particularly, biopolymers, and viruses.

United States Patent 1191 Kiselev et al.

[ June 10, 1975 1 1 PROCESS FOR PREPARATION OF WIDE-PORE ADSORBENT FOR USE IN CHROMATOGRAPHY [76] Inventors: Andrei Vladimirovich Kiselev,

Leninskie Gory, MGU, Zona M, kv. 160, Moscow; Galina Lvovna Kustova, ulitsa Burevestnika, 9, kv. 9; Boris Alexandrovich Lipkind, ulitsa Burevestnika, 9, kv. 3, both of Gorky; Jury Stepanovich Nikitin, Leningradskoe shosse, 120, korpus 3, kv. 4, Moscow, all of USSR.

22 Filed: May 11,1972

1211 Appl. No.: 252,462

OTHER PUBLICATIONS Book, The Colloid Chemistry of Silica and Silicates, by Ralph K. Iler, 1955 Ed., pages 137 and 138. Cornell Univ. Press, Ithaca, New York.

Primary Examiner-Edward Stern 5 7 ABSTRACT A process for preparing a wide-pore adsorbent adapted for use in chromatography, comprising mixing finely dispersed non-porous silica having a specific surface area S of 35 to 380 m /g with water followed by drying the aqueous suspension at a temperature within the range of 100 to 450C to give a silica xerogel and particulating thereof. The xerogel, prior to particulation or thereafter, is subjected to the hydrothermal treatment in an autoclave at a temperature within the range of from 100 to 380C and under a pressure of 1 to 300 atm., followed by drying at a temperature of 100 to 300C.

The above-described process makes possible the preparation of an adsorbent with larger pores as compared to adsorbents prepared from finely dispersed non-porous silica and produced by conventional processes. The process according to the present invention makes it possible to produce an adsorbent having a specific surface area within the range of from 2 to 280 m /g and an average pore diameter of from 230 to 30,000 A, while retaining a high pore volume of 0.9 to 1.9 em /g. Such adsorbent is useful in chromatography for the purposes of separation, purification and analysis of a wide range of substances: from lowand moderately-boiling compounds up to polymers, and more particularly, biopolymers, and viruses.

2 Claims, N0 Drawings PROCESS FOR PREPARATION OF WIDE-FORE ADSORBENT FOR USE IN CHROMATOGRAPHY The present invention relates to processes for the production of a wide-pore adsorbent adapted for use in chromatography from a finely dispersed non-porous silica having a specific surface area of 35 to 380 m lg. Such an adsorbent in useful as an active packing in chromatographic columns and as a support of stationary phases in gas and liquid chromatography, as well as a molecular sieve in gelpermeation chromatography.

A process is known for preparing a wide-pore adsorbent comprising mixing a finely dispersed non-porous silica having a specific surface area S of 170 or 280 m /g with water, followed by drying the suspension at a temperature of 100 to 450C to produce a silica xerogel. Said xerogel is then subjected to grinding and screening (cf. N. K. Bebris, A. V. Kiselev, Y. S. Nikitin Kolloidnyj zhournal 29, No. 3, 326, 1967; N. K. Bebris et al. Neftechimiajournal, 8, No. 3, 481, 1968).

The adsorbent produced by the known process has a specific surface area of 160 or 260 m /g and an average pore diameter of 550 and 230 A respectively.

Said prior-art process has a disadvantage in that it is impossible to increase the pore size of the resulting adsorbent within broad limits, thereby restricting its applicability in chromatography. Thus, for the analysis of high-molecular substances by methods of gelpermeation chromatography a range of adsorbent with various pore sizes is required including those with pores larger than in the adsorbent produced by the known process.

Another disadvantage of said process resides in great losses of the adsorbent during grinding thereof to particles less than 0.05 mm in size (up to percent by weight of the starting silica).

Still another disadvantage of the said process resides in the irregular shape of the particles resulting from grinding, whereby the uniformity of distribution of the chromatographic column packing particles and hydrodynamic properties of the packing are impaired.

It is an object of the present invention to eliminate the aforesaid disadvantages.

More particularly, it is an object of the present invention to change the manner of treatment of a xerogel produced by drying an aqueous suspension of a finely dispersed non-porous silica having a specific surface area S of 35 to 380 m /g at a temperature of 100 to 450C in order to prepare an adsorbent possessing the necessary structural characteristics and reduce its losses during the preparation thereof.

According to the present invention, said object has been accomplished by subjecting the xerogel of silica prior to particulation or thereafter to hydrothermal treatment in an autoclave at a temperature of lOO to 380 C and under a pressure of l to 300 atm., followed by drying thereof at a temperature of 100 to 300C.

The process of the present invention enables the preparation of an adsorbent with larger pores and smaller specific surface as compared to the adsorbent produced by the known process. By the hydrothermal treatment of the xerogel produced from non-porous silica it is possible to prepare an adsorbent having a specific surface area of from 2 to 280 m /g and an average pore diameter of from 230 to 30,000 A while retaining a high pore volume of from 0.9 to 1.9 cm /g. Such an adsorbent is useful in chromatography for separation,

purification, and analysis of a wide range of substances: from lowand moderately-boiling substances up to polymers, more particularly biopolymers, and viruses.

As has been mentioned above, the hydrothermal treatment of the silica xerogel may be effected both prior to and after the particulation of the xerogel. In doing so, the particulation of the xerogel is performed by grinding thereof.

In addition, in case of hydrothermal treatment of the xerogel followed by the drying thereof after the particulation procedure, it is advisable to effect said procedure simultaneously with drying the silica suspension by spraying thereof in a drying oven at a temperature of 400-450C. As a result of such combination of stages (suspension drying and xerogel particulation) the resulting xerogel is in the form of spherical particles which are much less liable to destruction in the autoclave during the hydrothermal treatment, this being especially important under high pressure and temperature treatment conditions. in addition, drying the suspension and particulation .of xerogel under the abovespecified conditions enables the obviation of xerogel grinding and is conducive to a reduction of adsorbent losses to as low as 5 to 7 percent by weight of the starting silica. The production of the adsorbent in the form of spherical particles also provides conditions for improving the uniformity of distribution of the chromatographic column packing particles and hydrodynamic properties of the packing.

For making the adsorbent applicable within a wide temperature range, for removing bound water therefrom and for increasing the adsorbent strength, it is advisable, prior to the hydrothermal treatment and/or after the hydrothermal treatment and drying of the silica xerogel, to calcine said xerogel at a temperature of 550 to l,0OOC in air or in a steam atmosphere.

The process for the preparation of a wide-pore adsorbent from finely dispersed non-porous silica and adapted for use in chromatography, according to the present invention, is effected in the following manner.

1 part by weight of finely dispersed silica (such as aerosil, white soot) is mixed with 1.6-12 parts by weight of water till a suspension is formed which is dried at a temperature of to 450C. The resulting silica xerogel is placed into an autoclave, mixed with water and subjected to hydrothermal treatment at a temperature of 100 to 380C under a pressure of l to 300 atm. during a period of 4 to 100 hours. After the hydrothermal treatment the xerogel is dried at a temperature'of 100 to 300C. The resulting adsorbent is then ground and screened into fractions. Grinding may also be performed prior to the hydrothermal treatment of the xerogel (after drying the aqueous suspension of silica).

To produce a wide-pore adsorbent in the form of spherical particles and reduce the adsorbent losses it is advisable to perform drying of the aqueous suspension of silica and the particulation of xerogel simultaneously by spraying said suspension in a drying oven at a temperature of 400 to 450C. The resulting xerogel particles have a spherical form and their size ranges from 0.05 to 1 mm. Then the hydrothermal treatment of the xerogel and its drying are effected is accordance with the above-described procedure, whereafter the adsorbent is ready for use.

As it has been mentioned hereinbefore, in order to make the adsorbent applicable within a wider temperature range, to remove bound water therefrom and to increase the adsorbent strength, it is advisable, prior to the hydrothermal treatment and/or after the hydrothermal treatment and drying of the xerogel of silica, to calcine said xerogel at a temperature of 550 to 1,000C in air or in a steam atmosphere during a period of 6 to 24 hours.

For a better understanding of the present invention the following examples of the preparation of a widepore adsorbent from a fine disperse non-porous silica are given by way of illustration.

EXAMPLE 1 280 g of finely dispersed non-porous silica having a specific surface area S of 175 m /g are mixed with one liter of water to yield a homogeneous suspension which is then dried at a temperature of 140C. The resulting xerogel is divided into three samples, each of them is subjected to hydrothermal treatment in an autoclave under the following conditions: sample I, at 100C and 1 atm. pressure for 69 hours; sample [1, at 275C and 60 atm. for 4 hours; sample 111, at 380C and 300 atm. for 4 hours. Then the samples are dried in a drying cabinet at 200C, ground and screened to separate the 0.05-0.8 mm fraction.

The yield of the final product for each of the samples is about 85 percent by weight of the starting silica.

The structural characteristics of the adsorbents produced from the three xerogel samples are given in Table 1 below.

mm fraction and calcined in air at 750C for 10 hours. The calcined xerogel is subjected to hydrothermal treatment in an autoclave at a temperature of 250C under a pressure of atm. for a period of 4 hours. Then the xerogel is dried at 200C and screened to separate the 0.05 to 0.5 mm fraction.

The yield of the final adsorbent is 92 percent by weight of the starting silica. The adsorbent has the following characteristics: specific surface area S 33 m /g; average pore diameter d= 1,650 A; pore volume V 1.37 cm /g.

EXAMPLE 4 280 g of finely dispersed non-porous silica having a specific surface area S of 175 m /g are mixed with one liter of water to produce a homogeneous suspension which is dried at a temperature of 140C. The resulting xerogel is hydrothermally treated in an autoclave at a temperature of 275C and under a pressure of 60 atm. for four hours. Thereafter the xerogel is dried at 200C and divided into two samples. Sample 1 is calcined in air at 1,000C for 6 hours, while sample 2 is calcinated in an atmosphere of steam at 750C for six hours. The calcined samples are ground and screened to separate the 0.05 to 0.8 mm fraction.

The yield of the final product for each of the samples is 84 percent by weight of the starting silica.

The structural characteristics of the adsorbent prepared from the two xerogel samples are given in Table 2 below.

Table 1 Structural characteris- Adsorbent Adsorbent Adsorbent tics of the resulting from from from adsorbent Sample 1 Sample 11 Sample lll Specific surface area, S m /g 135 48 4 Average pore diameter, d, A 560 1,600 19,500 Pore volume, V, cm /g 1.9 1.9 1.9

EXAMPLE 2 Table 2 Into an impeller 60 kg of finely dispersed non-porous St t l h t Ad b t Ad b t silica having a specific surface area of 380 m lg are f sg zg fs Es Fa added along with 490 1 of water and thoroughly 1nter- Sample 1 Sample 2 mixed. The resultmg suspension 1s dried in a spraying Specific Surface area S mzlg 45 47 furnace at a temperature of 450 C with a simultaneous Average pore d r, d, A 1,600 1,620

Pore volume V, cm"/g 1.8 1.9

particulation of the xerogel. 62 kg of the particulated xerogel featuring spherical particles of 0.05 to 0.8 mm in size and humidity of 5 percent is subjected to hydrothermal treatment in an autoclave at a temperature of 220C under 20 atm. pressure for 24 hours. Then the xerogel is dried at 300C for 8 hours, whereafter it is screened to obtain the 0.05 to 0.8 mm fraction.

The yield of the desired product is 57.9 kg or 96.5 percent by weight of the starting silica. The adsorbent has the following structural characteristics: specific surface area S =66 m /g; average pore diameter d 940 A; pore volume V= 1.55 em /g.

EXAMPLE 3 280 g of finely dispersed non-porous silica having a specific surface area S of 160 m /g are mixed with one liter of water to yield a homogeneous suspension which is dried at a temperature of 200C. The resulting xerogel is ground and screened to separate the 0.05 to 0.5

Said calcination of samples results in essential reduction of the bound water content W (umol H O/g of adsorbent) and hydroxyl group concentration on the adsorbent surface 04 (mcmol Ol-l/m of adsorbent surface). Thus, the adsorbent before the calcination has: W 630 and a 8.0; Sample 1 has: W= 30 and a a 1.3; Sample 2 has: W and 04 5.7. The calcination enhanced the adsorbent strength. The losses at vibration abrasion of the adsorbent prior to the calcination are 4.5 percent by weight, while those of the calcined samples 1 and 2 are 2.5 and 1.8 percent by weight respectively.

EXAMPLE 5 Into an impeller 60 kg of finely dispersed non-porous silica having a specific surface area S of 380 m /g and 490 l of water are charged and thoroughly intermixed. The resulting suspension is dried in a spraying furnace at a temperature of 450C with a simultaneous particulation of the xerogel. 62 kg of the particulated xerogel featuring spherical particles 0.05-0.8 mm in size and 5 percent humidity are subjected to hydrothermal treatment in an autoclave at a temperature of 220C and under a pressure of 20 atm, for a period of 24 hours. Then the xerogel is dried at 120C and calcined at 900C in air for a period of six hours. After the calcination the adsorbent is screened to separate the 0.05-0.8 mm fraction.

The yield of the final product is 57.6 kg or 96 percent by weight of the initial non-porous silica. The structural characteristics of the adsorbent are as follows. Specific surface area S 64 m /g; average pore diameter d 950 A; pore volume V 1.52 em /g.

EXAMPLE 6 Into an impeller 60 kg of finely dispersed non-porous silica having a specific surface area S of 175 m /g and 430 l of water are charged and thoroughly intermixed. The resulting suspension is dried in a spraying furnace at a temperature of 400C with simultaneous particulation of the xerogel. The particulated xerogel featuring spherical particles of 0.1 to 1 mm in size is divided into three samples; each of them is hydrothermally treated under the following conditions. Sample 1, 220C and 20 atm. for 96 hours; Sample 2, 280C and 100 atm. for 16 hours; Sample 3, 370C and 290 atm. for 6 hours. The samples are then dried at a temperature of 100C and calcinedin air at 900C over a period of 24 hours. After the calcination the samples are screened to separate the 0.1 to 1 mm fraction.

The yield of the final product produced from Sample 1 is 96 percent; Sample 2, 94.8 percent; Sample 3, 93 percent by weight of the starting silica.

The structural characteristics of the adsorbents produced from the three samples of the xerogel are given in Table below.

45 g of finely dispersed non-porous silica having a specific surface area S of 35 m /g are mixed with 100 ml of water. The resulting aqueous suspension is dried at a temperature of 100C in a drying cabinet. The xerogel thus obtained is hydrothermally treated at 100C and under a pressure of 3 atm. for a period of 4 hours. Then the xerogel is dried at 300C and calcined in air at 750C for eight hours. After the calcination the adsorbent is ground and screened to separate the 0.05 to 0.5 mm fraction.

The yield of the final product is 84 percent by weight of the starting silica. The adsorbent has the following structural characteristics: specific surface area S 29 m /g; average pore diameter d 1,250 A; pore volume V 0.92 cm lg.

EXAMPLE 8 Into an impeller 60 kg of finely dispersed non-ferrous silica having a specific surface area S of 175 m /g are charged along with 430 l of water and thoroughly intermixed. The resulting suspension is dried in a spraying furnace at a temperature of 400C with a simultaneous particulation of xerogel. The particulated xerogel featuring spherical particles of 0. 1-1 mm in size and 5 percent humidity is calcined in air at a temperature of 550C for a period of eight hours. The calcinated xerogel is then hydrothermally treated in an autoclave at a temperature of 220C and under 20 atm. pressure for a period of 96 hours. Thereafter, the xerogel is dried at 120C and calcined in the air at 900C for 24 hours. After the calcination the adsorbent is screened to separate the 0.1 to 1 mm fraction.

The yield of the final product is 98 percent by weight of the starting silica. The adsorbent has the following structural characteristics: specific surface area S 44 m /g average pore diameter d= 1,500 A; pore volume V= 1.68 cm /g.

EXAMPLE 9 Into an apparatus impeller 60 kg of finely dispersed silica having a specific surface area S of 175 m /g are charged along with 450 l of water and thoroughly intermixed. The resulting suspension is dried in a spraying furnace at a temperature of 450C with simultaneous particulation of xerogel. 62 kg of the particulated xerogel featuring spherical particles of 0.1 to 1 mm in size and 5 percent moisture content are calcined in an atmosphere of steam at 850C for a period of six hours.

The calcinated xerogel is hydrothermally treated at a temperature of 115C and under a pressure of 1.5 atm. for a period of 4 hours. Then the xerogel is dried at 300C and screened to separate the 0.1 1 mm fraction.

The yield of the final product is 97.5 percent by weight of the starting silica. The adsorbent has the following structural characteristics: specific surface area S m /g, average pore diameter d 32 700 A; pore volume V= 1.52 em /g.

We claim:

1. A process of preparing a porous silica adsorbent adapted for use in chromatography comprising the steps of (1) mixing finely dispersed non-porous silica having a specific surface area of 35 to 380 m /g with water to form an aqueous suspension; (2) spray drying the suspension at a temperature of 400 to 450C. to form a particulated silica xerogel; (3) hydrothermally treating the xerogel at a temperature of to 380C. and a pressure of l to 300 atm.; and (4) drying the treated xerogel at a temperature of 100 to 300C.

2. A process according to claim 1 further comprising the step of calcining the silica xerogel in an atmosphere of steam or air at a temperature of from 550 to 1000C. after either or both of steps (2) and (4). 

1. A PROCESS OF PREPARING A POROUS SILICA ADSORBENT ADAPTED FOR USE IN CHROMATORGRAPHY COMOMPRISING THE STEPS OF (1) MIXING FINELY DISPERSED NON-POROUS SILICA HAVING A SPECIFIC SURFACE AREA OF 35 TO 380 M2/G WITH WATER TO FORM AN AQUEOUS SUSPENSION; (2) SPRAY DRYING THE SUSPENSION AT A TEMPERATURE OF 400* TO 450*C. TO FORM A PARTICULATED SILICA XEROGEL; (3) HYDROTHERMALLY TREATING THE XEROGEL AT A TEMPERATURE OF 100* TO 380*C. AND A PRESSURE OF 1 TO 300 ATM.; AND (4) DRYING THE TREATED XEROGEL AT A TEMPERATURE OF 100* TO 300*C.
 2. A process according to claim 1 further comprising the step of calcining the silica xerogel in an atmosphere of steam or air at a temperature of from 550* to 1000*C. after either or both of steps (2) and (4). 